Is propidium iodide soluble in water?

Solubility : Soluble in water (1 mg/ml), DMSO, and methanol (3 mg/ml). Melting Point : 220-225° C (dec.)

What is the molecular nature of propidium iodide?

Propidium Iodide is a fluorescent nucleic acid dye which binds only to double-stranded nucleic acids. It has a molecular weight of 668.4, an absorbance maximum of 535nm, and an emission maximum of 617 nm. It is commonly used to determine the DNA content of a cell or to discriminate viable from non-viable cells.

Is propidium iodide toxic?

CONSIDERED A HAZARDOUS SUBSTANCE ACCORDING TO OSHA 29 CFR 1910.1200. Harmful if swallowed. Possible risk of irreversible effects. Irritating to eyes, respiratory system and skin.

How do you make fluorescein diacetate?

1. To prepare a FDA stock solution 5 mg of fluorescein diacetate is dissolved in 1 ml acetone.
2. 2,5 ml cell culture medium is cooled on ice.
3. 50 ml of the FDA stock solution is added and this diluted FDA solution is stored on ice.

How do you dissolve propidium iodide?

To make a stock solution from the solid form, dissolve PI (MW = 668.4) in deionized water (dH2O) at 1 mg/mL (1.5 mM) and store at 4°C, protected from light. When handled properly, solutions are stable for at least 6 months.

How do you dilute propidium iodide?

To prepare, add 1 mg PI (Propidium Iodide) to 1 ml distilled water. Store stock solution at 4 ºC (or aliquot and store at -20 ºC), protecting from light. When handled properly, solutions are stable for many years.

How does propidium iodide staining work?

Propidium iodide (or PI) is a fluorescent intercalating agent that can be used to stain cells and nucleic acids. PI binds to DNA by intercalating between the bases with little or no sequence preference. PI also binds to RNA, necessitating treatment with nucleases to distinguish between RNA and DNA staining.

How do you dissolve fluorescein diacetate?

Dissolve 0.00199 g of FDA in 200 mL of acetone in a glass bottle and cover with aluminum foil.

How do you reconstitute propidium iodide?

Reconstitute propidium iodide powder with 3.0 mL of PBS to a final concentration of 0.33 mg/mL. The reconstituted stock solution should be stored at -20°C and is stable for approximately 6 months.

What is propidium iodide?

Propidium Iodide is a fluorescent nucleic acid dye which binds only to double-stranded nucleic acids. It has a molecular weight of 668.4, an absorbance maximum of 535nm, and an emission maximum of 617 nm.

How do you prepare propidium iodide solution for flow cytometry?

Immediately before flow cytometric analysis, prepare a 0.5 mg/mL solution of propidium iodide by combining 50μL of 2X PBS with 50 μL of P4864. Add this solution to 1 mL of a cell suspension containing 10,000,000 cells.

How does propidium iodide detect dead cells?

Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population. PI binds to DNA by intercalating between the bases with little or no sequence preference.

Can You stain nucleic acids with propidium iodide?

Fluorescent stain for nucleic acids. Cell membrane integrity excludes propidium iodide from staining viable and apoptotic cells. Propidium iodide may be used in flow cytometry to evaluate cell viability when used with other dyes that stain viable cells or cells that are early in the apoptosis process.