How will you calibrate a microscope?

Calibrating a Microscope. To properly calibrate your reticle with a stage micrometer, align the zero line (beginning) of the stage micrometer with the zero line (beginning) of the reticle. Now, carefully scan over until you see the lines line up again.

Do microscopes get calibrated?

In order to estimate cell size during microscope investigations, the microscope should be calibrated. This means using a stage micrometer (a microscopic ruler), a calibration grid to measure the distance across each field of view (FOV1). As you increase the magnification the FOV decreases.

What is calibration in Micrometry?

This is a simple and precise method for measuring objects seen in the microscope. Ocular micrometers are calibrated by comparing the ocular micrometer scale with a calibrated stage micrometer. A calibration procedure must be completed to determine the calibration factor for each objective and each microscope.

How long is a stage micrometer?

2 mm long
A stage micrometer is simply a microscope slide with a scale etched on the surface. A typical micrometer scale is 2 mm long and at least part of it should be etched with divisions of 0.01 mm (10 µm). Suppose that a stage micrometer scale has divisions that are equal to 0.1 mm, which is 100 micrometers (µm).

How do you calibrate a microscope with an ocular micrometer?

Line up the micrometer with your eyepiece reticle scale. Place the stage micrometer on the stage of the microscope and bring the scale into focus. Position the stage micrometer so that its zero overlaps with the zero on the eyepiece reticle scale, as I’ve shown here. Onto the third step of calibrating your objectives.

What are the three rules in carrying the microscope?


  • Do not touch the glass part of the lenses with your fingers. Use only special lens paper to clean the lenses.
  • Always keep your microscope covered when not in use.
  • Always carry a microscope with both hands. Grasp the arm with one hand and place the other hand under the base for support.

What is microscope service and calibration?

Microscope Service and Calibration All microscope manufacturers strongly recommend regularly scheduled microscope service by a highly-trained microscope technician. Regularly scheduled care and maintenance is vital in maintaining performance and longevity of these delicate, highly relied upon instruments.

Where can I get an on-site microscope service?

At PCS, we provide on-site microscope services to customers whose facilities are located in Tennessee, Virginia, Alabama and Kentucky. If your company is near one of the following locations, we can perform your Microscope service or calibration on-site:

How do I calibrate the reticle of my microscope?

In order to calibrate your reticle follow the following procedure. 1. Switch to the lowest magnification of your microscope. 2. Place the stage micrometer onto your microscope with the 0 position on the stage micrometer in line with the 0 position of the reticle (eyepiece micromerter) as seen below: 3.

Why is it important to take care of Your Microscope?

Regularly scheduled care and maintenance is vital in maintaining performance and longevity of these delicate, highly relied upon instruments. Our experienced technicians will perform microscope service and calibration that includes cleaning, alignment, lens inspection and comparison to NIST calibrated stage micrometer.