What color is Alexa Fluor 594?
red-fluorescent
Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines.
Is quinine a fluorophore?
The first well-defined small-molecule fluorophore was the natural product quinine (1), an important compound for both medicinal and organic chemistry (15). These colored synthetic molecules were fodder for new biological experiments, and many found diagnostic or even clinical utility (21).
What is fluorophore made of?
Biological fluorophores are commonly comprised of fluorescent proteins such as GFP. They have been used for cell labeling and characterization with varied rates of success. Samples stained with GFP emit bright green fluorescent signals when excited with ultraviolet incident light.
Is APC a fluorophore?
Spectrum [APC (Allophycocyanin)] APC (Allophycocyanin) is a fluorescent compound with an excitation peak at 651 nm and an emission peak at 660 nm.
Is APC a good fluorophore?
APC is maximally excited at 652 nm (with a secondary maximum at 625 nm) and emits at 658 nm. Its fluorescence falls in the red region of the visible spectrum. Compared to other red fluorophores such as Cy5, however, APC is still significantly brighter and an excellent choice for use in flow cytometry.
What is Fluorochrome and fluorophore?
As nouns the difference between fluorochrome and fluorophore is that fluorochrome is any of various fluorescent dyes used to stain biological material before microscopic examination while fluorophore is (biochemistry) a molecule or functional group which is capable of fluorescence.
Which fluorochrome is used in tandem dyes?
The tandem dyes APC-Vio 770, APC-Cy7, and APC-H7 all use APC as the donor fluorochrome, showing maximum absorption around 652 nm. Emission spectra for Vio 770, Cy7, H7, and Alexa Fluor 750 are similar.
What are APC tandem dyes?
APC tandem dyes, APC-Cy5.5 and APC-Cy7, are also available. APC has a 650-nanometer wavelength absorption maximum and a 660-nanometer fluorescence emission maximum. APC can be used in flow cytometers equipped with dual lasers for multi-color analysis.
How many chromophores are there in APC?
APC has 6 phycocyanobilin chromophores per molecule, that are similar in structure to phycoerythrobilin, the chromophore in phycoerythrin (PE). APC tandem dyes, APC-Cy5.5 and APC-Cy7, are also available.
Can APC be used with a flow cytometer?
APC can be used in flow cytometers equipped with dual lasers for multi-color analysis. Like Alexa Fluor 633, APC is excited using the helium-neon red diode laser (633 nanometers) of the FACSCalibur and is detected using the FL4 detector.