Which culture medium is used for Mycobacterium What is its composition?

Lowenstein-Jensen (LJ) is the selective medium which is used for the cultivation and isolation of Mycobacterium species….Lowenstein-Jensen (LJ) Medium- Composition, Principle, Uses, Preparation and Colony Morphology.

Ingredients Amount
Magnesium Citrate 0.6 gm
Malachite Green 0.4 gm

What is a biphasic media?

Diphasic /biphasic medium is called so because of containing medium both solid and liquid states in a single bottle. It uses mainly for minimizing contamination and spread of infections. It applies to cultures of highly infectious agents like Brucella and Mycobacterium.

What are the components of the Lowenstein Jensen medium?


  • Malachite green.
  • Glycerol.
  • Asparagine.
  • Potato starch.
  • Coagulated eggs.
  • Mineral salt solution. Potassium dihydrogen phosphate. Magnesium sulfate. Sodium citrate.

What type of media is Lowenstein?

Löwenstein-Jensen medium, better known as LJ medium, is a selective egg-based medium specifically used for the culture and isolation of Mycobacterium species, including Mycobacterium tuberculosis, from clinical specimens.

What is Mgit culture?

MGIT stands for Mycobacteria Growth Indicator Tube. It is a system that determines whether or not TB bacteria will grow in the presence of TB drugs. The MGIT is a so-called liquid culture system. It has been developed by a company called Becton Dickinson (BD) in the United States.

What is biphasic growth?

A bacterial growth curve in which cultured bacteria have 2 exponential growth phases separated by a plateau phase.

Which media is used for blood culture?

Identification of the growth: To identify the organism, the blood has to be sub-cultured on to the solid medium. Commonly used media are the McConkey’s agar and the blood agar.

What agar is used for Mycobacterium tuberculosis?

Agar-based and egg-based media incorporating green malachite and Middlebrook broths or solid media are recommended as the “gold standard” for isolation, culture, and definite diagnosis of M. tuberculosis (6).

Which bacteria is isolated or grown using Lowenstein Jensen media?

Lowenstein Jensen (LJ) Media is a selective medium that is commonly used for the cultivation and isolation of Mycobacterium, specifically Mycobacterium tuberculosis from clinical specimens.

Is bactec and Mgit same?

When the combination with the solid medium was considered, the difference between the BACTEC MGIT 960 system and the BACTEC 460 system was further reduced. Most of the differences among various methods were for mycobacteria other than M. tuberculosis.

What is seven H11 agar?

Seven H11 Agar (also referred to as Middlebrook 7H11 Agar) was developed by Cohn et al. by the addition of casein hydrolysate to 7H10 Agar.1Seven H11 Agar provides enhanced User Quality Control

What is Middlebrook 7H10 agar?

Middlebrook 7H10 Agar is a defined medium consisting of oleic acid-albumin enrichment, glycerol, dextrose and inorganic compounds to supply the nutrients necessary to support the growth of mycobacterial species. Catalase destroys toxic peroxides that may be present in the medium.

What is the cat number for mycobacteria 7H11 agar?

Difco™Mycobacteria 7H11 Agar Cat. No. 283810 Dehydrated – 500 g BBL™Seven H11 Agar Base Cat. No. 212203 Dehydrated – 500 g BBL™Seven H11 Agar BS12 CMPH2 MCM9

What is Composition English language?

English Language Learners Definition of composition : the way in which something is put together or arranged : the combination of parts or elements that make up something : a piece of writing especially : a brief essay written as a school assignment : a written piece of music and especially one that is very long or complex